Extraction of gamete nuclei
Single-cell genome sequencing of haploid gametes and haplotype phasing
Genetic map construction based on the recombination patterns in the gamete genomes
Long-read sequencing of somatic material
Separation if long reads based on genetic linkage groups using phased alleles
Independent assembly of each haplotype of each linkage group
Scaffolding assemblies to chromosome-level using gamete-derived genetic map