a Extraction of gamete nuclei
b Single-cell genome sequencing of haploid gametes and haplotype phasing
c Genetic map construction based on the recombination patterns in the gamete genomes
d Long-read sequencing of somatic material
e Separation if long reads based on genetic linkage groups using phased alleles
f Independent assembly of each haplotype of each linkage group
g Scaffolding assemblies to chromosome-level using gamete-derived genetic map