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We develop methods to reconstruct genomes, find differences between them and try to understand what their contribution to phenotypic differences is.
Our group´s major focus is to advanced NGS-based analyses by addressing questions that could not be resolved previously. Whole-genome sequencing-based methods were used to accelerate forward genetics by directly linking mutant phenotypes to the genetic changes and to understand how genomes change over time (e.g. due to controlled mechanisms like recombination during meiosis).
Extraction of gamete nuclei
Single-cell genome sequencing of haploid gametes and haplotype phasing
Genetic map construction based on the recombination patterns in the gamete genomes
Long-read sequencing of somatic material
Separation if long reads based on genetic linkage groups using phased alleles
Independent assembly of each haplotype of each linkage group
Scaffolding assemblies to chromosome-level using gamete-derived genetic map